Anti-Caspase-9 Antibody | Boster A00080 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Caspase-9 Antibody

catalog :

A00080

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

A00080

status :

Enabled

name :

Anti-Caspase-9 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

CASP9

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Each vial contains 0.09% sodium azide.

description :

Polyclonal antibody for CASPASE 9/CASP9 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. CASPASE 9/CASP9 information: Molecular Weight: 46281 MW; Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

short description :

Rabbit Anti-Caspase-9 Antibody for CASP9 detection tested with WB in Human, Mouse, Rat.

size :

100 ug/vial

uniprot id :

P55211

host :

Rabbit

immunogen :

Recombinant human procaspase-9 (N-terminal fragment).

form :

Liquid

purification :

Protein A affinity purified.

application details :

WB, 1:500, ECL, Human, Mouse, Rat.

applications :

reactivity :

Human, Mouse, Rat

image labels :

Western blot analysis of Caspase-9 expression in untreated Jurkat cell lysates (lane 1), and apoptosisinduced Jurkat cell lysates (lane 2). Caspase-9 at 35/37kD, 18kD, 10kD was detected using rabbit anti- Caspase-9 Antigen Affinity purified polyclonal antibody (Catalog # A00080) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

background :

CASP9(CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates(GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.

competitor equivalent skus :

sc 22182 sc 8355 sc 133109 sc 7885 sc 17784 sc 8297 sc 56077 sc 73548 sc 56076 sc 70506 sc 70505 sc 81589 sc 81663 sc 70507 sc 81650 sc 56073

research category :

Apoptosis, Apoptotic Markers, Cancer, Caspases Etc, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes

synonyms :

Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6;

gene full name :

Caspase-9

molecular weight :

46281 MW

protein function :

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).

tissue specificity :

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

protein name :

Caspase-9 (CASP9)

last modified :

2/6/19 22:57