antibody

Anti-AKT1 Picoband Antibody

A00024

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

mouse, rat

Enabled

Anti-AKT1 Picoband Antibody

Primary Antibodies, Polyclonal Antibodies

Akt1

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Polyclonal antibody for Akt1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Mouse. Akt1 information: Molecular Weight: 55707 MW; Subcellular Localization: Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin- linked protein kinase 1 (ILK1) (By similarity). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles; Tissue Specificity: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.

Rabbit IgG polyclonal antibody for RAC-alpha serine/threonine-protein kinase(Akt1) detection. Tested with WB in Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P31750

Rabbit

A synthetic peptide corresponding to a sequence at the C-terminus of mouse AKT1 (397-421aa EDAKEIMQHRFFANIVWQDVYEKKL), different from the related human sequence by two amino acids, and identical to the related rat sequence.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot, 0.1-0.5ug/ml, Mouse, Rat.

WB

Mouse, Rat

Western blot analysis of AKT1 expression in rat kidney extract (lane 1) and NIH3T3 whole cell lysates (lane 2). AKT1 at 60KD was detected using rabbit anti- AKT1 Antigen Affinity purified polyclonal antibody (Catalog # A00024) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

sc 33549 sc 11160 sc 11158 sc 33550 sc 11155 sc 11156 sc 32331 sc 393436 sc 16698 sc 96 sc 34259 sc 34260 sc 6084 sc 217 sc 95 sc 366230 sc 514583 sc 8312 sc 56878 sc 81434 sc 1619 sc 1619 R sc 1619 X sc 271149 sc 1618 sc 5298 sc 55523 sc 7126 sc 377457 sc 135829 sc 1618 R

Apoptosis, Cancer, Cell Cycle, Cell Death, Epigenetics And Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Pathways And Processes, Protein Phosphorylation, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction

RAC-alpha serine/threonine-protein kinase;2.7.11.1;AKT1 kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;Thymoma viral proto-oncogene;Akt1;Akt, Rac;

RAC-alpha serine/threonine-protein kinase

55707 MW

AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)- response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr- 117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation (By similarity). Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity (By similarity). Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53 (By similarity).

Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin- linked protein kinase 1 (ILK1) (By similarity). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles.

Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.

RAC-alpha serine/threonine-protein kinase

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

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