antibody

Anti-STAT3 Antibody Picoband‚Ñ¢

A00007

10 ug sample

99 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

1

Anti-STAT3 Antibody Picoband‚Ñ¢

anti-stat3-picoband-trade-antibody-a00007-boster

Primary Antibodies

STAT3

315

10 ug sample

99 USD

Full size

315

Full size carrier free

365

Polyclonal

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Boster Bio Anti-STAT3 Antibody Picoband catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Boster Bio Anti-STAT3 Antibody Picoband catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

100μg/vial

P40763

Rabbit

E.coli-derived human STAT3 recombinant protein (Position: A2-R215). Human STAT3 shares 100% amino acid (aa) sequence identity with both mouse and rat STAT3.

Lyophilized

Immunogen affinity purified.

Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

WB

Human,Mouse,Rat

/antibody/a00007-1-WB-anti-stat3-picoband-antibody.jpg

Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (A00007). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates . Lane 2: mouse brain tissue lysates . Lane 3: mouse testis tissue lysates . Lane 4: COLO320 whole cell lysates. After Electrophoresis proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # A00007) at 0.5 μg/mL overnight at 4°C then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88KD. The expected band size for STAT3 is at 88KD.

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1/22/17 14:08

3/2/22 2:55

Anti-STAT3 Antibody Picoband Bosterbio A00007

Anti-STAT3 Antibody Picoband

Boster Bio Anti-STAT3 Antibody Picoband catalog # A00007. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

100

Signal transducer and activator of transcription 3 (STAT3) is a transcription factor which in humans is encoded by the STAT3 gene. It is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

sc 102129 sc 169533 sc 135426 sc 135425 sc 373835 sc 376864 sc 21345 R sc 398997 sc 21344 R sc 15708 sc 15709 sc 244139 sc 244138 sc 244137 sc 18644 sc 25479 sc 166805 sc 13511 sc 658 sc 365395 sc 166776 sc 18645 sc 25480 sc 483 sc 7179 sc 8019 sc 482 sc 81001 sc 293151 sc 483 X sc 7179 X sc 8019 X sc 482 X

Cancer,Cardiogenesis,Cardiovascular,Developmental Biology,Embryogenesis,Embryonic Stem Cells,Epigenetics And Nuclear Signaling,Hypertrophy,Intracellular,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,Stem Cells,Surface Molecules,Transcription,Transcription Factors,Transcription Factors/Regulators

Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF;

Signal transducer and activator of transcription 3

88068 MW

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Signal transducer and activator of transcription 3

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

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