rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-E Cadherin antibody, PA2078, Western blotting. Lane 1: A549 Cell Lysate. Lane 2: SGC Cell Lysate.
quantity: 100 ug/vial
price: 240 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Anti- E Cadherin Picoband antibody, PB9561, Western blotting. All lanes: Anti E Cadherin (PB9561) at 0.5ug/ml. Lane 1: Human Placenta Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Predicted bind size: 140KD. Observed bind size: 140KD
Anti- E Cadherin Picoband antibody, PB9561, IHC(P). IHC(P): Human Placenta Tissue
Anti- E Cadherin Picoband antibody, PB9561, IHC(F). IHC(F): Human Placenta Tissue
quantity: 100 ug/vial
price: 280 USD
to the supplier
mouse monoclonal (9G2)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. IF analysis of COL4A1/E Cadherin using anti- COL4A1/E Cadherin antibody (PB9099/M00063-2) . COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Boster Kit Box
quantity: 100 ug/vial
price: 280 USD
to the supplier
rabbit monoclonal (COH-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Immunohistochemical analysis of paraffin-embedded colon cancer, using pan Cadherin Antibody (M00063-1). CDH1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDH1 Antibody (M00063-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of pan Cadherin expression in C6 cell lysate (M00063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH1 monoclonal antibody (Catalog # M00063-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH1
quantity: 100 ug/vial
price: 299 USD
to the supplier