domestic rabbit polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry
reactivity: human, bovine
application: western blot, immunohistochemistry
Anti-CDC42 antibody, PA1366, Western blotting. Lane 1: Recombinant Human CDC42 Protein 10ng. Lane 2: Recombinant Human CDC42 Protein 5ng. Lane 3: Recombinant Human CDC42 Protein 2.5ng
Anti-CDC42 antibody, PA1366, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (IAE-3)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
Western blot analysis of CDC42 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate (M00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # M00119) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (IBB-3)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of Phospho-Rac1/Cdc42 (Ser71) expression in (1) A431 cell lysate treated with LP; (2) A431 cell lysate (P00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # P00119) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section
Figure 1. IHC analysis of CDC42 using anti-CDC42 antibody (A00119). CDC42 was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of CDC42 using anti-CDC42 antibody (A00119). CDC42 was detected in paraffin-embedded section of human mammary cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry