mouse monoclonal (DCS-193)
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Anti- Cdc25C Picoband antibody, PB9756, Western blotting. All lanes: Anti Cdc25C (PB9756) at 0.5ug/ml. Lane 1: Rat Ovary Tissue Lysate at 50ug. Lane 2: Rat Liver Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 60KD. Observed bind size: 60KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of Cdc25C expression in HELA whole cell lysates (lane 1), SW620 whole cell lysates (lane 2) and MCF-7 whole cell lysates (lane 3). Cdc25C at 53KD was detected using rabbit anti- Cdc25C Antigen Affinity purified polyclonal antibody (Catalog # PB9849) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (HIG-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of Cdc25C expression in K562 cell lysate (M01343). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25C monoclonal antibody (Catalog # M01343) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25C
quantity: 100 µl
price: 315 USD
to the supplier