Anti-CDK1 Antibody (Monoclonal, A17)
Boster
catalog: MA1077

mouse monoclonal (A17)
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry

Boster Kit Box
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CDK1 Antibody (Monoclonal, PSTAIR)
Boster
catalog: MA1087

mouse monoclonal (PSTAIR)
reactivity: human, mouse, rat
application: western blot

Boster Kit Box
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Cyclin-dependent kinase 1 CDK1 Antibody
Boster
catalog: PA1544

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-CDK1 antibody, PA1544, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: 293T Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: JURKAT Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CDK1 Antibody Picoband™
Boster
catalog: PB9533

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti- CDK1 Picoband antibody, PB9533, Western blotting. All lanes: Anti CDK1 (PB9533) at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50ug. Lane 2: Rat Spleen Tissue Lysate at 50ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: NIH3T3 Whole Cell Lysate at 40ug. Predicted bind size: 34KD. Observed bind size: 34KD

Anti- CDK1 Picoband antibody, PB9533, IHC(P). IHC(P): Mouse Testis Tissue

Anti- CDK1 Picoband antibody, PB9533, IHC(P). IHC(P): Rat Testis Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Boster
catalog: M00209-2

domestic rabbit monoclonal (BOH-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human colon, using CDK1 Antibody(M00209-2). CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (M00209-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody
Boster
catalog: P00209-1

domestic rabbit monoclonal (DFH-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody(P00209-1). CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (P00209-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea (P00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # P00209-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CDK1 Antibody Picoband™ (monoclonal, 2G11)
Boster
catalog: M00209-6

mouse monoclonal (2G11)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Boster
catalog: M00209-1

domestic rabbit monoclonal (AAD-3)
reactivity: human
application: western blot, immunoprecipitation

Western blot analysis of CDK1 in HeLa cell lysate (M00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Boster
catalog: M00209-3

domestic rabbit monoclonal (BOI-3)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-3) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Boster
catalog: M00209-4

domestic rabbit monoclonal (CBB-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (M00209-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-4) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
quantity: 100 µl
price: 315 USD
to the supplier