Anti-CD81 Rabbit Monoclonal Antibody
Boster
catalog: M01281-1

domestic rabbit monoclonal (GAA-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of CD81 expression in Ramos cell lysate (M01281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD81 monoclonal antibody (Catalog # M01281-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD81

IHC analysis of paraffin-embedded section of human tonsil tissues using anti-CD81 antibody (M01281-1). CD81 was detected in paraffin-embedded. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD81 Antibody (M01281-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-TAPA1/CD81 Antibody Picoband™
Boster
catalog: A01281-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). TAPA1 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (A01281-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. Flow Cytometry analysis of PBMC cells using anti-TAPA1 antibody (A01281-2). Overlay histogram showing PBMC cells stained with A01281-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2,1ug/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 3. Flow Cytometry analysis of Jurkat cells using anti-TAPA1 antibody (A01281-2). Overlay histogram showing Jurkat cells stained with A01281-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2,1ug/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Human TAPA1 DyLight® 488 conjugated CD81 Antibody
Boster
catalog: A01281-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human TAPA1 DyLight® 550 conjugated CD81 Antibody
Boster
catalog: A01281-Dyl550

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-CD81 antigen CD81 Antibody
Boster
catalog: A01281

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-Human CD81 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
Boster
catalog: FC01281

mouse monoclonal (1D6)
reactivity: human
application: flow cytometry

quantity: 100 ug, 500 ug
price: 147 USD
to the supplier

Anti-Human CD81 Monoclonal Antibody APC Conjugated, Flow Validated
Boster
catalog: FC01281-APC

mouse monoclonal (1D6)
reactivity: human
conjugate: APC
application: flow cytometry

quantity: 100 Tests, 200 Tests
price: 325 USD
to the supplier

Anti-Human CD81 Monoclonal Antibody FITC Conjugated, Flow Validated
Boster
catalog: FC01281-FITC

mouse monoclonal (1D6)
reactivity: human
conjugate: FITC
application: flow cytometry

quantity: 100 Tests, 200 Tests
price: 235 USD
to the supplier

Anti-Human CD81 Monoclonal Antibody PE Conjugated, Flow Validated
Boster
catalog: FC01281-PE

mouse monoclonal (1D6)
reactivity: human
conjugate: PE
application: flow cytometry

quantity: 100 Tests, 200 Tests
price: 279 USD
to the supplier