Anti-CD44 Antibody Picoband™
Boster
catalog: PB9333

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti- CD44 Picoband antibody, PB9333, Western blotting. All lanes: Anti CD44 (PB9333) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PANC Whole Cell Lysate at 40ug. Predicted bind size: 82KD. Observed bind size: 115KD

Anti- CD44 Picoband antibody, PB9333, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Figure 3. IF analysis of CD44 using anti-CD44 antibody (PB9333) . CD44 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD44 Rabbit Monoclonal Antibody
Boster
catalog: M00052-1

domestic rabbit monoclonal (AGI-3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of CD44 expression in TF-1 cells lysate (M00052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 monoclonal antibody (Catalog # M00052-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44

Immunohistochemical analysis of paraffin-embedded human skin, using CD44 Antibody(M00052-1). CD44 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (M00052-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CD44 antigen CD44 Antibody
Boster
catalog: PA1021-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Anti-CD44 antibody, PA1021-2, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-CD44 antibody, PA1021-2, IHC(P). IHC(P): Rat Intestine Tissue

Anti-CD44 antibody, PA1021-2, ICC. ICC: HCT116 Cell
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Human CD44 Monoclonal Antibody FITC Conjugated, Flow Validated
Boster
catalog: FC00052-FITC

mouse monoclonal (Hermes-3)
reactivity: human
conjugate: FITC
application: flow cytometry

Lymphocytes gated PBMCs stained with FITC conjugated anti-human CD44 (clone Hermes-3) (red histogram). Lymphocytes gated PBMCs stained with FITC conjugated mouse IgG2a isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by using FlowJo software.
quantity: 100 Tests, 200 Tests
price: 235 USD
to the supplier

Anti-CD44 Antibody Picoband™
Boster
catalog: A00052

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (A00052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,. Lane 2: human 22RV1 cell lysate,. Lane 3: rat spleen tissue lysate,. Lane 4: rat thymus tissue lysate,. Lane 5: mouse spleen tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # A00052) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 88KD. The expected band size for CD44 is at 82KD.

Figure 2. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Phospho-CD44 (S706) Antibody
Boster
catalog: A00052S706

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Human CD44 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
Boster
catalog: FC00052

mouse monoclonal (Hermes-3)
reactivity: human
application: flow cytometry

quantity: 100 ug, 500 ug
price: 147 USD
to the supplier

Anti-Human CD44 Monoclonal Antibody PE Conjugated, Flow Validated
Boster
catalog: FC00052-PE

mouse monoclonal (Hermes-3)
reactivity: human
conjugate: PE
application: flow cytometry

quantity: 100 Tests, 200 Tests
price: 279 USD
to the supplier

Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264
Boster
catalog: M00052-2

domestic rabbit monoclonal (RM264)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier

Anti-CD44 Picoband™ Antibody (monoclonal, 7H7)
Boster
catalog: M00052-3

mouse monoclonal (7H7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 100µg/vial
price: 315 USD
to the supplier