Anti-CD40L/CD40LG Antibody
Boster
catalog: PA1020

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of sCD40L using anti- sCD40L antibody (PA1020). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate,. Lane 2: HELA Whole Cell Lysate,. Lane 3: JURKAT Whole Cell Lysate,. Lane 4: HMY2 Whole Cell Lysate,. Lane 5: COLO320 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- sCD40L antigen affinity purified polyclonal antibody (Catalog # PA1020) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for sCD40L at approximately 29KD. The expected band size for sCD40L is at 29KD.

Figure 2. IHC analysis of sCD40L using anti- sCD40L antibody (PA1020). sCD40L was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- sCD40L Antibody (PA1020) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of sCD40L using anti- sCD40L antibody (PA1020). sCD40L was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- sCD40L Antibody (PA1020) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD40L/Cd40lg Antibody Picoband™
Boster
catalog: A01114-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 36KD. The expected band size for CD40L is at 29KD.

Figure 2. IHC analysis of CD40L using anti-CD40L antibody (A01114-2). CD40L was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD40L Antibody (A01114-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD40L using anti-CD40L antibody (A01114-2). CD40L was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD40L Antibody (A01114-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CD40L CD40LG Rabbit Monoclonal Antibody
Boster
catalog: M01114

domestic rabbit monoclonal (AAIE-3)
reactivity: human, mouse
application: western blot

Western blot analysis of CD40L expression in MOLT-4 cell lysate (M01114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG monoclonal antibody (Catalog # M01114) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40LG
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CD40L/CD40LG Antibody Picoband™
Boster
catalog: A01114-1

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CD40L/CD40LG Antibody Picoband™
Boster
catalog: A01114-3

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of CD40LG using anti-CD40LG antibody (A01114-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG antigen affinity purified polyclonal antibody (Catalog # A01114-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40LG at approximately 36KD. The expected band size for CD40LG is at 29KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CD40L CD40LG Antibody
Boster
catalog: A01114

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-TRAP/CD40L/CD40LG Antibody Picoband™
Boster
catalog: A01114-4

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Ms CD154 Purified CD40LG Monoclonal Antibody
Boster
catalog: M01114-1

hamsters monoclonal (MR-1)
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 0.1 mg (C100)
price: 280 USD
to the supplier