domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of GDF15 using anti- GDF15 antibody (A01583-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 22RV whole Cell lysates, . Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- GDF15 antigen affinity purified polyclonal antibody (Catalog # A01583-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDF15 at approximately 34KD. The expected band size for GDF15 is at 34KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
mouse monoclonal (monoclonal)
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit monoclonal (20G21)
reactivity: human
application: western blot
reactivity: human
application: western blot