domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
Anti-CD34 antibody, PA1334, Western blotting. Lane 1: Recombinant Human CD34 Protein 10ng. Lane 2: Recombinant Human CD34 Protein 5ng. Lane 3: Recombinant Human CD34 Protein 2.5ng
Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Rat Kidney Tissue
Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Human Lung Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (BBH-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Immunohistochemical analysis of paraffin-embedded human kidney, using CD34 Antibody(M00885-1). CD34 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD34 Antibody (M00885-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of CD34 in expression NIH/3T3 cell lysate (M00885-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 monoclonal antibody (Catalog # M00885-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
domestic rabbit monoclonal (RM300)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section