domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit polyclonal
reactivity: human, rat
application: ELISA, immunohistochemistry
reactivity: human, rat
application: ELISA, immunohistochemistry
Figure 1. IHC analysis of CD80 using anti-CD80 antibody (A00196-1). CD80 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD80 Antibody (A00196-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of CD80 using anti-CD80 antibody (A00196-1). CD80 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD80 Antibody (A00196-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
domestic rabbit monoclonal (AOEH-3)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
mouse monoclonal (8G12)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, flow cytometry