Anti-CD163 Antibody
Boster
catalog: PA1874

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-CD163 antibody, PA1874, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human CD163, 35.2KD (162aa tag+E1002-L1156). Lane 1: Recombinant Human CD163 Protein 10ng . Lane 2: Recombinant Human CD163 Protein 5ng . Lane 3: Recombinant Human CD163 Protein 2.5ng
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD163 Antibody Picoband™
Boster
catalog: A00812-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (A00812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human placenta tissue lysates,. Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # A00812-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150KD. The expected band size for CD163 is at 128KD.

Figure 2. IHC analysis of CD163 using anti-CD163 antibody (A00812-1). CD163 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD163 using anti-CD163 antibody (A00812-1). CD163 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CD163 Antibody
Boster
catalog: PA1874-1

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-CD163 antibody, PA1874-1, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-CD163 antibody, PA1874-1, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-CD163 antibody, PA1874-1, Western blotting. All lanes: Anti CD163 (PA1874-1) at 0.5ug/ml. Lane 1: Recombinant Human CD163 Protein 10ng. Lane 2: Recombinant Human CD163 Protein 5ng. Lane 3: Recombinant Human CD163 Protein 2.5ng. Predicted bind size: 35KD. Observed bind size: 35KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Human CD163 DyLight® 488 conjugated Antibody
Boster
catalog: A00812-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human CD163 DyLight® 550 conjugated Antibody
Boster
catalog: A00812-Dyl550

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-CD163 Antibody Picoband™
Boster
catalog: A00812-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-CD163 Rabbit Monoclonal Antibody
Boster
catalog: M00812

domestic rabbit monoclonal (ABCC-3)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

quantity: 100 µl
price: 315 USD
to the supplier

Anti-CD163 Rabbit Monoclonal Antibody, Clone#RM371
Boster
catalog: M00812-1

domestic rabbit monoclonal (RM371)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier