Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated
Boster
catalog: FC02236

mouse monoclonal (OKT8)
reactivity: human, mouse
application: flow cytometry

quantity: 100 ug, 500 ug
price: 114 USD
to the supplier

Anti-CD8 alpha/CD8A Antibody Picoband™
Boster
catalog: PB9249

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti- CD 8 alpha Picoband antibody, PB9249, Western blotting. All lanes: Anti CD 8 alpha (PB9249) at 0.5ug/ml. WB: Recombinant Human CD 8 alpha Protein 0.5ng. Predicted bind size: 29KD. Observed bind size: 29KD

Anti- CD 8 alpha Picoband antibody, PB9249, Western blotting. All lanes: Anti CD 8 alpha (PB9249) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: RAJI Whole Cell Lysate at 40ug. Lane 3: HL-60 Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Predicted bind size: 26KD. Observed bind size: 55KD

Anti- CD 8 alpha Picoband antibody, PB9249, IHC(P). IHC(P): Human Tonsil Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD8 alpha Rabbit Monoclonal Antibody
Boster
catalog: M02236-2

domestic rabbit monoclonal (BDG-3)
reactivity: human
application: immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human spleen, using CD8 alpha Antibody(M02236-2). CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-2)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CD8 CD8A Rabbit Monoclonal Antibody
Boster
catalog: M02236-1

domestic rabbit monoclonal (EAF-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody(M02236-1). CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD8 expression in Jurkat cell lysate (M02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8A monoclonal antibody (Catalog # M02236-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8A
quantity: 100 µl
price: 315 USD
to the supplier

Anti-human CD8 Cd8a Monoclonal Antibody FITC Conjugated, Flow Validated
Boster
catalog: FC02236-FITC

mouse monoclonal (OKT8)
reactivity: human, mouse
conjugate: FITC
application: flow cytometry

quantity: 100 Tests, 200 Tests
price: 132 USD
to the supplier

Anti-CD8 Alpha CD8A Antibody
Boster
catalog: A02236

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 697 USD
to the supplier