mouse monoclonal (OKT4)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
rabbit monoclonal (DOE-3)
reactivity: human
application: western blot, immunocytochemistry
reactivity: human
application: western blot, immunocytochemistry
Western blot analysis of CD4 expression in THP-1 cell lysate (M00344). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 monoclonal antibody (Catalog # M00344) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4
quantity: 100 ug/vial
price: 299 USD
to the supplier
mouse monoclonal (CA-4)
reactivity: human, rat
application: immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human, rat
application: immunohistochemistry, immunocytochemistry, flow cytometry
mouse monoclonal (OKT4)
reactivity: human
conjugate: APC
application: flow cytometry
reactivity: human
conjugate: APC
application: flow cytometry
Lymphocytes gated PBMCs stained with APC conjugated mouse anti-human CD4 (clone OKT-4, red histogram). PBMCs lymphocytes stained with isotype IgG2b APC (blue histogram)
quantity: 25 Tests, 100 Tests, 200 Tests
price: 69 USD
to the supplier
mouse monoclonal (OKT4)
reactivity: human
conjugate: PE
application: flow cytometry
reactivity: human
conjugate: PE
application: flow cytometry
Lymphocytes gated PBMCs stained with PE conjugated anti-human CD4 (clone OKT-4, red histogram). PBMCs lymphocytes stained with isotype IgG2b PE (blue histogram)
quantity: 25 Tests, 100 Tests, 200 Tests
price: 54 USD
to the supplier