Anti-Histone H3 Antibody
Boster
catalog: A12477

rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity: 100 uL
price: 329 USD
to the supplier

Anti-Histone H3 Antibody
Boster
catalog: A15033

rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 ul
price: 200 USD
to the supplier

Anti-H3K4me3 Antibody
Boster
catalog: CI1001

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K9me3 Antibody
Boster
catalog: CI1002

rabbit polyclonal
reactivity: human
application: western blot, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K4me3 Antibody ChIP Grade
Boster
catalog: CI1004

rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K4me2 Antibody
Boster
catalog: CI1006

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K4me1 Antibody
Boster
catalog: CI1007

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K79me2 Antibody
Boster
catalog: CI1009

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K9me3 Antibody
Boster
catalog: CI1010

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K36me3 Antibody
Boster
catalog: CI1012

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K9me1 Antibody
Boster
catalog: CI1013

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K79me3 Antibody ChIP Grade
Boster
catalog: CI1014

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K79me1 Antibody
Boster
catalog: CI1015

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K27me3S28p Antibody
Boster
catalog: CI1017

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3R17me2 (asym) Antibody
Boster
catalog: CI1018

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3S10p Antibody
Boster
catalog: CI1022

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K36me2 Antibody
Boster
catalog: CI1023

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K9me3S10p Antibody
Boster
catalog: CI1024

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3pan Antibody
Boster
catalog: CI1025

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K18ac Antibody ChIP Grade
Boster
catalog: CI1026

rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K23ac Antibody ChIP Grade
Boster
catalog: CI1027

rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-H3K9ac Antibody
Boster
catalog: CI1029

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/37 ul
price: 380 USD
to the supplier

Anti-H3K9/14ac Antibody
Boster
catalog: CI1030

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/36 ul
price: 380 USD
to the supplier

Anti-H3K4me3 Antibody
Boster
catalog: CI1038

rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/25 ul
price: 380 USD
to the supplier

Anti-H3K4me2 Antibody
Boster
catalog: CI1040

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/42 ul
price: 380 USD
to the supplier

Anti-H3K4me1 Antibody
Boster
catalog: CI1041

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/18 ul
price: 380 USD
to the supplier

Anti-H3K27me1 Antibody
Boster
catalog: CI1043

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/82 ul
price: 380 USD
to the supplier

Anti-H3K27me2 Antibody
Boster
catalog: CI1044

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/68 ul
price: 380 USD
to the supplier

Anti-H3K79me2 Antibody
Boster
catalog: CI1046

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/46 ul
price: 380 USD
to the supplier

Anti-H3K9me3 Antibody
Boster
catalog: CI1049

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/33 ul
price: 380 USD
to the supplier

Anti-H3K36me3 Antibody
Boster
catalog: CI1051

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/56 ul
price: 380 USD
to the supplier

Anti-H3K9me2 Antibody
Boster
catalog: CI1052

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/44 ul
price: 380 USD
to the supplier

Anti-H3K9me1 Antibody
Boster
catalog: CI1054

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 50 ug/43 ul
price: 380 USD
to the supplier

Anti-H3K79me3 Antibody
Boster
catalog: CI1055

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/42 ul
price: 380 USD
to the supplier

Anti-H3K27me3 Antibody
Boster
catalog: CI1056

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/34 ul
price: 380 USD
to the supplier

Anti-H3K79me1 Antibody
Boster
catalog: CI1059

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/31 ul
price: 380 USD
to the supplier

Anti-H3K36me1 Antibody
Boster
catalog: CI1064

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 50 ug/54 ul
price: 380 USD
to the supplier

Anti-H3K27me3S28p Antibody
Boster
catalog: CI1065

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/ 295 ul
price: 380 USD
to the supplier

Anti-H3S10p Antibody
Boster
catalog: CI1067

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/48 ul
price: 380 USD
to the supplier

Anti-H3K18ac Antibody
Boster
catalog: CI1068

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/62 ul
price: 380 USD
to the supplier

Anti-H3K4ac Antibody
Boster
catalog: CI1070

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/34 ul
price: 380 USD
to the supplier

Anti-H3R17me2(asym)K18ac Antibody
Boster
catalog: CI1072

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/53 ul
price: 380 USD
to the supplier

Anti-H3K27ac Antibody
Boster
catalog: CI1073

rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/42 ul
price: 380 USD
to the supplier

Anti-H3K9ac Antibody
Boster
catalog: CI1074

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/47 ul
price: 380 USD
to the supplier

Anti-H3K4me3 Antibody
Boster
catalog: CI1075

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 50 ul
price: 380 USD
to the supplier

Anti-H3K64me3 Antibody
Boster
catalog: CI1076

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation

quantity: 50 ug/ 84 ul
price: 380 USD
to the supplier

Anti-H3K56ac Antibody
Boster
catalog: CI1077

rabbit polyclonal
reactivity: human
application: western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/ 84 ul
price: 380 USD
to the supplier

Anti-H3pan Antibody ChIP Grade
Boster
catalog: CI1089

rabbit polyclonal
reactivity: human
application: ELISA, immunocytochemistry

quantity: 100 ul
price: 250 USD
to the supplier

Anti-H3pan Antibody ChIP Grade
Boster
catalog: CI1126

rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity: 50 ug/53 ul
price: 250 USD
to the supplier

Anti-H3K9ac Antibody (Monoclonal)
Boster
catalog: CI1130

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/50 ul
price: 295 USD
to the supplier

Anti-H3pan Antibody (Monoclonal)
Boster
catalog: CI1137

mouse monoclonal
reactivity: human
application: western blot, chromatin immunoprecipitation

quantity: 50 ug/100 ul
price: 380 USD
to the supplier

Anti-H3K27me2/3 Antibody (Monoclonal)
Boster
catalog: CI1138

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/34 ul
price: 380 USD
to the supplier

Anti-H3K4un Antibody (Monoclonal)
Boster
catalog: CI1139

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/50 ul
price: 380 USD
to the supplier

Anti-H3K4me1 Antibody (Monoclonal)
Boster
catalog: CI1140

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/50 ul
price: 380 USD
to the supplier

Anti-H3K4me2 Antibody (Monoclonal)
Boster
catalog: CI1141

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/50 ul
price: 380 USD
to the supplier

Anti-H3K4me3 Antibody (Monoclonal)
Boster
catalog: CI1142

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

quantity: 50 ug/50 ul
price: 380 USD
to the supplier

Anti-H3K27me3 Antibody (Monoclonal)
Boster
catalog: CI1146

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation

quantity: 50 ug/50 ul
price: 380 USD
to the supplier

Anti-Histone H3 (mono methyl R17) Rabbit Monoclonal Antibody
Boster
catalog: M12477-4

rabbit monoclonal (BDI-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Histone H3 (mono methyl R17) expression in HeLa cell lysate (M12477-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-4) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A

Immunohistochemical analysis of paraffin-embedded mouse colon, using Histone H3 (mono methyl R17) Antibody(M12477-4). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-4)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal Antibody
Boster
catalog: M12477-1

rabbit monoclonal (AFF-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4 . Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Methyl-Histone H3 (di K4) expression in HeLa cell lysate (M12477-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (acetyl K56) Rabbit Monoclonal Antibody
Boster
catalog: P12477-1

rabbit monoclonal (BAB-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 (acetyl K56) expression in C6 cell lysate, treated with TSA (P12477-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # P12477-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Phospho-Histone H3 (S10) Monoclonal Antibody
Boster
catalog: MP12477

rabbit monoclonal (BAE-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Figure 2. IHC analysis of HIST1H3A using anti-HIST1H3A antibody (MP12477). HIST1H3A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (MP12477) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of HIST1H3A using anti-HIST1H3A antibody (MP12477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A antigen affinity purified polyclonal antibody (Catalog # MP12477) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HIST1H3A.
quantity: 100 ug
price: 299 USD
to the supplier

Anti-Histone H3 (acetyl K14) Rabbit Monoclonal Antibody
Boster
catalog: P12477-2

rabbit monoclonal (BID-8)
reactivity: human, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Acetyl-Histone H3 (K14) expression in C6 cell lysate (P12477-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # P12477-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono methyl R128) Rabbit Monoclonal Antibody
Boster
catalog: M12477-7

rabbit monoclonal (8-Dec)
reactivity: human, mouse
application: western blot

Western blot analysis of Histone H3 (mono methyl R128) expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M12477-7). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-7) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono methyl K36) Rabbit Monoclonal Antibody
Boster
catalog: M12477-5

rabbit monoclonal (DEG-8)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 (mono methyl K36) expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M12477-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-5) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono methyl R2) Rabbit Monoclonal Antibody
Boster
catalog: M12477-3

rabbit monoclonal (DFB-8)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 (mono methyl R2) expression in HeLa cell lysate (M12477-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-3) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono+di+tri methyl K79) Rabbit Monoclonal Antibody
Boster
catalog: M12477-8

rabbit monoclonal (H-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Immunohistochemical analysis of paraffin-embedded rat colon, using Histone H3 (mono+di+tri methyl K79) Antibody(M12477-8). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-8)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Histone H3 (mono+di+tri methyl K79) expression in (1) NIH/3T3 cell lysate; (2) A549 cell lysate (M12477-8). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-8) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono methyl K18) Rabbit Monoclonal Antibody
Boster
catalog: M12477-6

rabbit monoclonal (DFC-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Histone H3 (mono methyl K18) expression in HeLa cell lysate (M12477-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-6) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A

Immunohistochemical analysis of paraffin-embedded human liver, using Histone H3 (mono methyl K18) Antibody(M12477-6). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-6)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (mono+di+tri methyl K14) Rabbit Monoclonal Antibody
Boster
catalog: M12477-9

rabbit monoclonal (I-8)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 (mono+di+tri methyl K14) expression in HeLa cell lysate (M12477-9). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-9) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (acetyl K4) Rabbit Monoclonal Antibody
Boster
catalog: P12477

rabbit monoclonal (DHD-8)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 (acetyl K4) expression in HeLa+TSA cell lysate (P12477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # P12477) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 (di methyl K9) Rabbit Monoclonal Antibody
Boster
catalog: M12477-2

rabbit monoclonal (GBI-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

Western blot analysis of Histone H3 (di methyl K9) expression in HeLa cell lysate (M12477-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 Rabbit Monoclonal Antibody
Boster
catalog: M12477-10

rabbit monoclonal (HDE-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (M12477-10). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-10) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Histone H3 Rabbit Monoclonal Antibody
Boster
catalog: M12477

rabbit monoclonal (IHA-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry

Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate (M12477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
quantity: 100 ug/vial
price: 299 USD
to the supplier