Anti-SQSTM1/p62 Antibody
Boster
catalog: PA1955

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-SQSTM1/p62 antibody, PA1955, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: A549 Cell Lysate.

Anti-SQSTM1/p62 antibody, PA1955, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-SQSTM1/p62 antibody, PA1955, ICC. ICC: Hela Cell
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SQSTM1/p62 Antibody Picoband™
Boster
catalog: PB9444

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Figure 1. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SQSTM1/p62 Antibody Picoband™ (monoclonal, 3H11)
Boster
catalog: M00300-1

mouse monoclonal (3H11)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
Boster
catalog: M00300

domestic rabbit monoclonal (EBB-19)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of p62/SQSTM1 expression in SKBR-3 cell lysate (M00300). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 monoclonal antibody (Catalog # M00300) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-SQSTM1 / p62 Rabbit Monoclonal Antibody
Boster
catalog: M00300-3

domestic rabbit monoclonal (21S51)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 µl/vial
price: 315 USD
to the supplier

Anti-Phospho-p62 (Ser28) SQSTM1 Antibody
Boster
catalog: P00300-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot

quantity: 100µl
price: 435 USD
to the supplier