domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry
reactivity: human, rat
application: western blot, immunohistochemistry
Anti- Neuropillin-1 Picoband antibody, PB9300, Western blotting. All lanes: Anti Neuropillin-1 (PB9300) at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: Human Placenta Tissue Lysate at 50ug. Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug. Predicted bind size: 103KD. Observed bind size: 103KD
Anti- Neuropillin-1 Picoband antibody, PB9300, IHC(P). IHC(P): Human Placenta Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (BGO-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of Neuropilin 1 expression in mouse heart lysate (M01324). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRP1 monoclonal antibody (Catalog # M01324) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRP1
Immunohistochemical analysis of paraffin-embedded human melanoma, using Neuropilin 1 Antibody (M01324). NRP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NRP1 Antibody (M01324)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
mouse monoclonal (4G3F7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
mouse monoclonal (8G6G7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunocytochemistry, flow cytometry