domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Western blot analysis of CASR expression in HELA whole cell lysates (lane 1) and 22RV1 whole cell lysates (lane 2). CASR at 150KD was detected using rabbit anti- CASR Antigen Affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
CASR was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- CASR Antigen Affinity purified polyclonal antibody (Catalog # PB9924) at 1 ??g/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
CASR was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- CASR Antigen Affinity purified polyclonal antibody (Catalog # PB9924) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (11000000000)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
Figure 2. Western blot analysis of CASR using anti-CASR antibody (M00574). Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,. Lane 2: human A549 whole cell lysate,. Lane 3: human 22RV1 whole cell lysate,. Lane 4: human HepG2 whole cell lysate,. Lane 5: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CASR antigen affinity purified monoclonal antibody (Catalog # M00574) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (11000000000)
reactivity: human
conjugate: DyLight488
application: flow cytometry
reactivity: human
conjugate: DyLight488
application: flow cytometry
mouse monoclonal (11000000000)
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
