Anti-Caspase-8 (P18)/CASP8 Antibody
Boster
catalog: PA1308

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti-Caspase-8(P18) antibody, PA1308, Western blotting. All lanes: Anti Caspase-8(P18) (PA1308) at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50ug. Lane 2: Rat Liver Tissue Lysate at 50ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 55KD. Observed bind size: 55KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Caspase-8(P10)/CASP8 Antibody
Boster
catalog: PA1524

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-Caspase-8(P10) antibody, PA1524, Western blotting. WB: HELA Cell Lysate

Anti-Caspase-8(P10) antibody, PA1524, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Caspase-8(p18)/CASP8 Antibody
Boster
catalog: PA1735

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti- Caspase-8(p18) antibody, PA1735, Western blotting. All lanes: Anti Caspase-8(p18) (PA1735) at 0.5ug/ml. Lane 1: A549 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Predicted bind size: 18KD. Observed bind size: 18KD

Anti- Caspase-8(p18) antibody, PA1735,IHC(P). IHC(P): Human Tonsil Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Caspase 8/CASP8 Antibody Picoband™
Boster
catalog: A00042-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.

Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, . Lane 2: mouse spleen tissue lysates, . Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55-60KD. The expected band size for Caspase 8 is at 55KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Caspase-8 Rabbit Monoclonal Antibody
Boster
catalog: M00042-2

domestic rabbit monoclonal (IB-3)
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of Caspase-8 expression in(1) Jurkat cell lysate; (2)HeLa cell lysate (M00042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP8 monoclonal antibody (Catalog # M00042-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP8
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
Boster
catalog: M00042

domestic rabbit monoclonal (EEG-3)
reactivity: human
application: western blot

Western blot analysis of Caspase-8 expression in Jurkat cell lysate (M00042). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP8 monoclonal antibody (Catalog # M00042) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP8
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
Boster
catalog: M00042-1

domestic rabbit monoclonal (GAI-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Caspase-8 expression in HeLa cell lysate (M00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP8 monoclonal antibody (Catalog # M00042-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP8
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-8(p18)/CASP8 Antibody Picoband™
Boster
catalog: A00042-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-Caspase-8/CASP8 Antibody Picoband™
Boster
catalog: A00042-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier