domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Figure 1. Western blot analysis of Caspase-6 using anti-Caspase-6 antibody (A02631). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,. Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6 antigen affinity purified polyclonal antibody (Catalog # A02631) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6 at approximately 33KD. The expected band size for Caspase-6 is at 33KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
Anti-Caspase-6(P18) antibody, PA1441, IHC(P). IHC(P): Human Mammary Cancer Tissue
Anti-Caspase-6(P18) antibody, PA1441, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: SW620 Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (EHB-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of Caspase-6 expression in Jurkat cell lysate (M02631-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (GBD-3)
reactivity: human
application: western blot, immunoprecipitation
reactivity: human
application: western blot, immunoprecipitation
Western blot analysis of Caspase-6 p18 expression in Jurkat cell treated with 1uM staurosporine lysate (M02631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (HAA-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of Caspase-6 expression in MCF-7 cell lysate (M02631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody (Catalog # M02631) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP6
quantity: 100 µl
price: 315 USD
to the supplier