Anti-Caspase 3/CASP3 Antibody
Boster
catalog: PA1849

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti- CASP3 antibody, PA1849, Western blotting. All lanes: Anti CASP3 (PA1849) at 0.5ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 2: Rat Liver Tissue Lysate at 50ug. Lane 3: Rat Thymus Tissue Lysate at 50ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: SMMC Whole Cell Lysate at 40ug. Lane 6: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 31KD. Observed bind size: 31KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Caspase-3/CASP3 Antibody Picoband™
Boster
catalog: PB9188

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Caspase3 using anti-Caspase3 antibody (PB9188). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Caspase3 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase3 at approximately 39KD. The expected band size for Caspase3 is at 39KD.

Figure 2. IHC analysis of Caspase3 using anti-Caspase3 antibody (PB9188). Caspase3 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase3 Antibody (PB9188) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Caspase3 using anti-Caspase3 antibody (PB9188). Caspase3 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase3 Antibody (PB9188) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334-3

domestic rabbit monoclonal (HE-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin (M00334-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-3) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3

IF analysis of immunocytochemical section of HepG2 cell using anti- active Caspase-3 antibody (M00334-3) . Active Caspase-3 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- active Caspase-3 Antibody (M00334-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334-4

domestic rabbit monoclonal (DGI-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody (M00334-4). CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-4)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Calreticulin expression in (1) Jurkat cell lysate; (2) COLO cells lysate (M00334-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-4) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-3 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334-5

domestic rabbit monoclonal (AOE-3)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Caspase-3 in HEK293 cell lysate (M00334-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-5) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3

Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody(M00334-5). CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-5)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-3 (P10)/CASP3 Antibody
Boster
catalog: PA1302-1

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Caspase-3(P10), PA1302-1, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-Caspase-3(P10), PA1302-1, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: SMMC Cell Lysate.

Anti-Caspase-3(P10), PA1302-1, IHC(P). IHC(P): Human Lung Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Human Caspase-3 DyLight® 488 conjugated CASP3 Antibody
Boster
catalog: A00334-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human Caspase-3 DyLight® 550 conjugated CASP3 Antibody
Boster
catalog: A00334-Dyl550

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Caspase-3 Antibody Picoband™ (monoclonal, 8B6)
Boster
catalog: M00334-6

mouse monoclonal (8B6)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)
Boster
catalog: M00334-7

mouse monoclonal (15G8)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334

domestic rabbit monoclonal (AOB-3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of pro Caspase 3 in HeLa cell lysate (M00334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
quantity: 100 µl
price: 315 USD
to the supplier

Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334-1

domestic rabbit monoclonal (GAG-3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of pro Caspase 3 expression in Jurkat cell lysate (M00334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal Antibody
Boster
catalog: M00334-2

domestic rabbit monoclonal (GEA-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate (M00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Caspase-3 CASP3 Antibody
Boster
catalog: A00334

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: ELISA, immunohistochemistry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-Caspase-3(P17)/CASP3 Antibody Picoband™
Boster
catalog: A00334-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Caspase-3(p17)/CASP3 Antibody Picoband™
Boster
catalog: A00334-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-Phospho-Caspase-3 (S150) CASP3 Antibody
Boster
catalog: A00334S150

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250
Boster
catalog: M00334-8

domestic rabbit monoclonal (RM250)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 399 USD
to the supplier