Anti-Calbindin Antibody
Boster
catalog: M03047-2

chicken polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1

Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, M03047-2, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. The M03047-2 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to calbindin, M03047-2, dilution 1:2,000, in green, and costained with rabbit pAb to MeCP2, dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.
quantity: 100 ul
price: 299 USD
to the supplier

Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955)
Boster
catalog: MA1007

mouse monoclonal (CB-955)
reactivity: human, mouse, rat, bovine
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Calbindin-D antibody (monoclonal), MA1007, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate. Lane 4: Rat Lung Tissue Lysate.

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P). IHC(P): Mouse Kidney Tissue

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P). IHC(P): Rat Kidney Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Calbindin/CALB1 Antibody Picoband™
Boster
catalog: PB9045

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). Calbindin was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). Calbindin was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Calbindin/CALB1 Antibody Picoband™
Boster
catalog: A03047

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Calbindin using anti-Calbindin antibody (A03047). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat kidney tissue lysates,. Lane 3: mouse brain tissue lysates,. Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody (Catalog # A03047) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calbindin at approximately 28KD. The expected band size for Calbindin is at 30KD.

Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (A03047). Calbindin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (A03047). Calbindin was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Calbindin CALB1 Rabbit Monoclonal Antibody
Boster
catalog: M03047

domestic rabbit monoclonal (AAAA-3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 µl
price: 315 USD
to the supplier

Anti-Calbindin Monoclonal Antibody
Boster
catalog: M03047-1

mouse monoclonal (monoclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 299 USD
to the supplier