Anti-CXCR4 Antibody Picoband™
Boster
catalog: A00031

domestic rabbit polyclonal
reactivity: human
application: western blot

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40KD. The expected band size for CXCR4 is at 40KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CXCR4 Antibody
Boster
catalog: PA1237

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, . Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.

Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CXCR4 Antibody
Boster
catalog: PA2081

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-CXCR4 antibody, PA2081, Western blotting. Lane 1: M231 Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: JURKAT Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CXCR4 Monoclonal Antibody
Boster
catalog: M00031

domestic rabbit monoclonal (CID-3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (M00031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # M00031) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CXCR4.

Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (M00031). CXCR4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (M00031) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CXCR4 Antibody Picoband™
Boster
catalog: A00031-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates,. Lane 2: human PC-3 whole cell lysates,. Lane 3: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 36-40KD. The expected band size for CXCR4 is at 40KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CXCR4 Antibody
Boster
catalog: A00031-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-CXCR4 Antibody Picoband™
Boster
catalog: A00031-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CXCR4 Antibody Picoband™
Boster
catalog: A00031-4

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CXCR4 (phospho-S339) Antibody
Boster
catalog: A00031S339

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 ul
price: 547 USD
to the supplier

Anti-Phospho-Fusin (S339) CXCR4 Antibody
Boster
catalog: A00031S339-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier