Anti-CRM1/XPO1 Antibody Picoband™
Boster
catalog: PB9966

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, . Lane 2: mouse brain tissue lysates, . Lane 3: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (Catalog # PB9966) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123KD. The expected band size for CRM1 is at 123KD.

Figure 2. IHC analysis of CRM1 using anti-CRM1 antibody (PB9966). CRM1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CRM1 using anti-CRM1 antibody (PB9966). CRM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CRM1 XPO1 Antibody Picoband™ (monoclonal, 5G3)
Boster
catalog: M01180

mouse monoclonal (5G3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: rat lung tissue lysates,. Lane 3: mouse liver tissue lysates, . Lane 4: mouse lung tissue lysates, . Lane 5: Rabbit IgG, . Lane 6: Marker 1113, . Lane 7: human HepG2 whole cell lysates, . Lane 8: human SMMC-7721 whole cell lysates, . Lane 9: human Hela whole cell lysates,. Lane 10: human JURKAT whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system."

Figure 2. IHC analysis of CRM1 using anti-CRM1 antibody (M01180). CRM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CRM1 Antibody (M01180) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. IHC analysis of CRM1 using anti-CRM1 antibody (M01180). CRM1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CRM1 Antibody (M01180) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3)
Boster
catalog: M01180-Dyl488

mouse monoclonal (5G3)
reactivity: human
conjugate: DyLight488
application: flow cytometry

quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3)
Boster
catalog: M01180-Dyl550

mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-CRM1 XPO1 Antibody
Boster
catalog: A01180

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-CRM1 Rabbit Monoclonal Antibody
Boster
catalog: M01180-1

domestic rabbit monoclonal (AEFD-24)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 µl
price: 315 USD
to the supplier