Anti-Vinculin/VCL Antibody Picoband™
Boster
catalog: A01207

domestic rabbit polyclonal
reactivity: human
application: western blot

Figure 1. Western blot analysis of Vinculin using anti- Vinculin antibody (A01207). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Vinculin antigen affinity purified polyclonal antibody (Catalog # A01207) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)
Boster
catalog: MA1103

mouse monoclonal (hVIN-1)
reactivity: human, mouse, rat, chicken
application: western blot, immunocytochemistry, immunohistochemistry - frozen section

Figure 1. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. Western blot analysis of Vinculin using anti-Vinculin antibody (MA1103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela, whole cell lysates, . Lane 2: human HepG2, whole cell lysates, . Lane 3: monkey COS-7 whole cell lysates, . Lane 4: human U-87MG whole cell lysates,. Lane 5: rat PC-12 whole cell lysates, . Lane 6: rat RH35 whole cell lysates, . Lane 7: mouse HEPA1-6 whole cell lysates, . Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Vinculin antigen affinity purified monoclonal antibody (Catalog # MA1103) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Vinculin/VCL Antibody
Boster
catalog: PA1781

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Anti-Vinculin antibody, PA1781, Western blotting. Lane 1: Rat Heart Tissue Lysate . Lane 2: Rat Brain Tissue Lysate . Lane 3: Rat Liver Tissue Lysate . Lane 4: U87 Cell Lysate . Lane 5: SMMC Cell Lysate . Lane 6: HEPA Cell Lysate . Lane 7: HELA Cell Lysate. Lane 8: HT1080 Cell Lysate

Anti-Vinculin antibody, PA1781, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-Vinculin antibody, PA1781, IHC(P). IHC(P): Rat Intestine Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Vinculin VCL Rabbit Monoclonal Antibody
Boster
catalog: M01207

domestic rabbit monoclonal (AIG-22)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate (M01207). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL monoclonal antibody (Catalog # M01207) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Vinculin/VCL Antibody Picoband™
Boster
catalog: A01207-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Vinculin VCL Antibody (biotin)
Boster
catalog: A01207-Biotin

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-Phospho-Vinculin (Y821) VCL Antibody
Boster
catalog: A01207Y821

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Vinculin VCL Antibody
Boster
catalog: A30448

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 323 USD
to the supplier

Anti-Vinculin VCL Monoclonal Antibody
Boster
catalog: M01207-1

mouse monoclonal (AS1A35)
reactivity: human
application: immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier