mouse monoclonal (BP53-12)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry

Figure 1. Western blot analysis of p53 using anti-p53 antibody (MA1078). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HL-60 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p53 antigen affinity purified monoclonal antibody (Catalog # MA1078) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p53 at approximately 53KD. The expected band size for p53 is at 53KD.

Figure 2. IHC analysis of p53 using anti-p53 antibody (MA1078). p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. IF analysis of p53 using anti- p53 antibody (MA1078) . p53 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL mouse anti- p53 Antibody (MA1078) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (Pab122)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Figure 3. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 1. Western blot analysis of Tp53 using anti-Tp53 antibody (M00001-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tp53 antigen affinity purified polyclonal antibody (Catalog # M00001-4) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Tp53.
quantity: 200µg
price: 470 USD
to the supplier
domestic rabbit monoclonal (BDO-20)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-p53 (Ser392) expression in HEK293 whole cell lysate (P00001-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody (Catalog # P00001-6) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53

Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody(P00001-6). TP53 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TP53 Antibody (P00001-6)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
Anti-p53 (phospho-S15) TP53 Antibody
Boster
catalog: A00001S15-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 547 USD
to the supplier
Anti-p53 (phospho-S20) TP53 Antibody
Boster
catalog: A00001S20
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-p53 (S20) TP53 Antibody
Boster
catalog: A00001S20-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (phospho-S315) TP53 Antibody
Boster
catalog: A00001S315
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-p53 (S315) TP53 Antibody
Boster
catalog: A00001S315-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-p53 (S366) TP53 Antibody
Boster
catalog: A00001S366
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (phospho-S37) TP53 Antibody
Boster
catalog: A00001S37
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-p53 (S37) TP53 Antibody
Boster
catalog: A00001S37-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (Phospho-S376) TP53 Antibody
Boster
catalog: A00001S376
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Phospho-p53 (S376) TP53 Antibody
Boster
catalog: A00001S376-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-p53 (S378) TP53 Antibody
Boster
catalog: A00001S378
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-p53 (S392) TP53 Antibody
Boster
catalog: A00001S392
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (phospho-S6) TP53 Antibody
Boster
catalog: A00001S6-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 547 USD
to the supplier
Anti-p53 (S9) TP53 Antibody
Boster
catalog: A00001S9-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Phospho-p53 (T18) TP53 Antibody
Boster
catalog: A00001T18
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (T310) TP53 Antibody
Boster
catalog: A00001T310
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Phospho-p53 (T387) TP53 Antibody
Boster
catalog: A00001T387
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 (Phospho-T55) TP53 Antibody
Boster
catalog: A00001T55
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Phospho-p53 (T81) TP53 Antibody
Boster
catalog: A00001T81
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-p53 TP53 Antibody
Boster
catalog: CI1060
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, chromatin immunoprecipitation, ChIP-Seq
quantity: 50µg/50µl
price: 420 USD
to the supplier
Anti-P53 Antibody
Boster
catalog: M00001
mouse monoclonal (monoclonal)
reactivity: human
application: western blot
quantity: 50 ul
price: 170 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
quantity: 100µl
price: 435 USD
to the supplier