Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody
Boster
catalog: M00002

mouse monoclonal (TNF706 + P/T2)
reactivity: human, mouse, rat, dogs
application: immunohistochemistry

Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained withTNF alpha Monoclonal Antibody (TNF706 + P/T2).
quantity: 100 ug/vial
price: 440 USD
to the supplier

Anti-TNF alpha Antibody
Boster
catalog: PA1079

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human placenta tissue lysates, . Lane 3: human U-937 whole cell lysates, . Lane 4: human HL-60 whole cell lysates. Lane 5: human A431 whole cell lysates, . Lane 6: human A549 whole cell lysates, . Lane 7: human K562 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 28KD. The expected band size for TNF alpha is at 25KD.

Figure 2. IHC analysis of TNF alpha using anti-TNF alpha antibody (PA1079). TNF alpha was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF alpha Antibody (PA1079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of TNF alpha using anti-TNF alpha antibody (PA1079). TNF alpha was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF alpha Antibody (PA1079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-TNF alpha Antibody Picoband™
Boster
catalog: A00002-3

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-TNF-Alpha (Q178) Antibody
Boster
catalog: A00002

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-TNF alpha Purified Azide Free Monoclonal Antibody
Boster
catalog: M00002-1

mouse monoclonal (MAb1)
reactivity: human
application: western blot, ELISA

quantity: 0.1 mg (C100)
price: 280 USD
to the supplier

Anti-TNF alpha Purified Monoclonal Antibody
Boster
catalog: M00002-2

mouse monoclonal (MAb11)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 0.1 mg (C100)
price: 280 USD
to the supplier

Anti-TNF alpha Monoclonal Antibody
Boster
catalog: M00002-3

mouse monoclonal (Q34)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier