domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of TIMP3 using anti- TIMP3 antibody (A00477). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, . Lane 2: NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TIMP3 antigen affinity purified polyclonal antibody (Catalog # A00477) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 24KD. The expected band size for TIMP3 is at 24KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Anti- TIMP3 Picoband antibody, PB9831, Western blotting. All lanes: Anti TIMP3 (PB9831) at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50ug. Lane 2: Mouse Ovary Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 34KD. Observed bind size: 34KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry
reactivity: human
application: immunohistochemistry