Anti-CD90/Thy1 Antibody
Boster
catalog: PA1515-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
domestic rabbit monoclonal (CEB-20)
reactivity: human, rat
application: western blot, immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human tonsil, using CD90 / Thy1 Antibody(M01818). THY1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-THY1 Antibody (M01818)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD90 expression in Human fetal brain lysate (M01818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THY1 monoclonal antibody (Catalog # M01818) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THY1
quantity: 100 µl
price: 315 USD
to the supplier
mouse monoclonal (F15-42-1-5)
reactivity: human
conjugate: APC
application: flow cytometry
quantity: 100 Tests, 200 Tests
price: 341 USD
to the supplier
mouse monoclonal (F15-42-1-5)
reactivity: human
conjugate: PE
application: flow cytometry
quantity: 100 Tests, 200 Tests
price: 319 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (F15-42-1-5)
reactivity: human
application: flow cytometry
quantity: 100 ug, 500 ug
price: 220 USD
to the supplier
mouse monoclonal (F15-42-1-5)
reactivity: human
conjugate: biotin
application: flow cytometry
quantity: 100µg
price: 267 USD
to the supplier
domestic rabbit monoclonal (AOFC-20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 µl
price: 315 USD
to the supplier