Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
Boster
catalog: MA1100
mouse monoclonal (TH-16)
reactivity: human, rat
application: western blot, immunohistochemistry

Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100). Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, . Lane 2: mouse brain tissue lysate, . Lane 3: human U-87MG cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Tyrosine Hydroxylase/TH Antibody Picoband™
Boster
catalog: PB9449
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Anti- Tyrosine Hydroxylase Picoband antibody, PB9449, Western blotting. All lanes: Anti Tyrosine Hydroxylase (PB9449) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Predicted bind size: 59KD. Observed bind size: 59KD

Anti- Tyrosine Hydroxylase Picoband antibody, PB9449,IHC(P). IHC(P): Mouse Brain Tissue

Anti- TH Picoband antibody, PB9449,IHC(P). IHC(P): Rat Brain Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
Boster
catalog: M01917
domestic rabbit monoclonal (GOO-20)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of Tyrosine Hydroxylase expression in PC12 cell lysate (M01917). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH monoclonal antibody (Catalog # M01917) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TH
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Tyrosine Hydroxylase TH Rabbit Monoclonal Antibody
Boster
catalog: M01917-1
domestic rabbit monoclonal (ECA-20)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Immunohistochemical analysis of paraffin-embedded human kidney, using Tyrosine Hydroxylase Antibody (M01917-1). TH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TH Antibody (M01917-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Tyrosine Hydroxylase expression in PC-3 cell lysate (M01917-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH monoclonal antibody (Catalog # M01917-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TH
quantity: 100 µl
price: 315 USD
to the supplier
Anti-TH Antibody (C-term)
Boster
catalog: A01917
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 80 ul
price: 166 USD
to the supplier
Anti-Phospho-TH (S19) Antibody
Boster
catalog: A01917S19
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-TH (S62) Antibody
Boster
catalog: A01917S62
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-TH (phospho-S71) Antibody
Boster
catalog: A01917S71
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-TH (S71) Antibody
Boster
catalog: A01917S71-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-TH (phospho-S8) Antibody
Boster
catalog: A01917S8
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-TH (S8) Antibody
Boster
catalog: A01917S8-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier