Anti-Estrogen Inducible Protein pS2/Tff1 Antibody Picoband™
Boster
catalog: A01391

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Tff1 using anti-Tff1 antibody (A01391). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse gaster tissue lysate,. Lane 2: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tff1 antigen affinity purified polyclonal antibody (Catalog # A01391) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tff1 at approximately 12KD. The expected band size for Tff1 is at 12KD.

Figure 2. IHC analysis of Tff1 using anti-Tff1 antibody (A01391). . Tff1 was detected in paraffin-embedded section of mouse gaster . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tff1 Antibody (A01391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Tff1 using anti-Tff1 antibody (A01391). . Tff1 was detected in paraffin-embedded section of rat gaster . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tff1 Antibody (A01391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. .
quantity: 100 ug/vial
price: 315 USD
to the supplier

Anti-Estrogen Inducible Protein pS2/TFF1 Antibody
Boster
catalog: PA1689

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-Estrogen Inducible Protein Ps2 antibody, PA1689, IHC(P). IHC(P): Human Gastric Cancer Tissue

Anti-Estrogen Inducible Protein Ps2 antibody, PA1689, Western blotting. WB: MCF-7 Cell Lysate
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband™
Boster
catalog: PB9522

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Anti- TFF1 Picoband antibody, PB9522, Western blotting. All lanes: Anti TFF1 (PB9522) at 0.5ug/ml. WB: HELA Whole Cell Lysate at 40ug. Predicted bind size: 12KD. Observed bind size: 12KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Trefoil factor 1 TFF1 Antibody
Boster
catalog: A01391-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 ul
price: 637 USD
to the supplier

Anti-Trefoil factor 1 TFF1 Antibody
Boster
catalog: A01391-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-TFF1 Antibody Picoband™
Boster
catalog: A01391-4

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-pS2 / pNR-2 / Trefoil Factor 1 (Estrogen-Regulated Protein) TFF1 Monoclonal ...
Boster
catalog: M01391

mouse monoclonal (SPM313)
reactivity: human
application: immunohistochemistry

quantity: 100 ug/vial
price: 440 USD
to the supplier

Anti-Estrogen Inducible Protein pS2 Monoclonal Antibody
Boster
catalog: M01391-1

domestic rabbit monoclonal (ADIB-20)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 µl
price: 315 USD
to the supplier

Anti-TFF1 Antibody (Center)
Boster
catalog: M01391-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry

quantity: 50 ul
price: 166 USD
to the supplier