Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)
Boster
catalog: MA1091

mouse monoclonal (SVP-38)
reactivity: human, rat
application: western blot, immunohistochemistry

Anti-Synaptophysin antibody (monoclonal), MA1091, Western blotting. WB: Rat Brain Tissue Lysate

Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P). IHC(P): Rat Brain Tissue

Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P). IHC(P): Human Glioma Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Synaptophysin/SYP Antibody Picoband™
Boster
catalog: PB9409

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of Synaptophysin using anti- Synaptophysin antibody (PB9409). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Synaptophysin antigen affinity purified polyclonal antibody (Catalog # PB9409) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
Boster
catalog: M05049

domestic rabbit monoclonal (BIE-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Immunohistochemical analysis of paraffin-embedded human kidney, using Synaptophysin Antibody (M05049). SYP was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SYP Antibody (M05049)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. Western blot analysis of Synaptophysin using anti- Synaptophysin antibody (M05049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Synaptophysin antigen affinity purified polyclonal antibody (Catalog # M05049) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 38KD.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
Boster
catalog: M05049-1

domestic rabbit monoclonal (BGB-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded mouse brain, using Synaptophysin Antibody (M05049-1). SYP was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SYP Antibody (M05049-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Synaptophysin expression in SH-SY5Y cell lysate (M05049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP monoclonal antibody (Catalog # M05049-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Synaptophysin/SYP Picoband™ Antibody
Boster
catalog: A05049

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-SYP/Synaptophysin Antibody Picoband™ (monoclonal, 3G12)
Boster
catalog: M05049-3

mouse monoclonal (3G12)
reactivity: human, mouse, rat
application: western blot

quantity: 100µg/vial
price: 315 USD
to the supplier