Anti-CD147/BSG Antibody
Boster
catalog: PA1526
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Anti-CD147 antibody, PA1526, Western blotting. WB: Human Fibroma Cell Lysate
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-CD147/BSG Antibody Picoband™
Boster
catalog: PB9247
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of CD147 using anti-CD147 antibody (PB9247). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CD147 Protein 0.5ng . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147 antigen affinity purified polyclonal antibody (Catalog # PB9247) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147 at approximately 39KD. The expected band size for CD147 is at 39KD.

Figure 2. Western blot analysis of CD147 using anti-CD147 antibody (PB9247). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Placenta Tissue Lysate. Lane 2: JURKAT Whole Cell Lysate. Lane 3: U20S Whole Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147 antigen affinity purified polyclonal antibody (Catalog # PB9247) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147 at approximately 42KD. The expected band size for CD147 is at 42KD.

Figure 3. Flow Cytometry analysis of Hela cells using anti- Emmprin antibody (PB9247). Overlay histogram showing Hela cells stained with PB9247 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Emmprin Antibody (PB9247,1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-CD147/Emmprin/BSG Antibody Picoband™
Boster
catalog: A00248-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of CD147/Emmprin using anti- CD147/Emmprin antibody (A00248-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Hela whole cell lysates,. Lane 2: Human Placenta whole cell lysates, . Lane 3: Human Jurkat whole cell lysates, . Lane 4: Human U20S whole cell lysates,. Lane 5: Human K562 whole cell lysates,. Lane 6: Human MCF-7 whole cell lysates,. Lane 7: Human COLO-320 whole cell lysates,. Lane 8: Human HepG2 whole cell lysates,. Lane 9: Human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD147/Emmprin antigen affinity purified polyclonal antibody (Catalog # A00248-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 38KD and 55KD. The expected band size for CD147/Emmprin is at 42KD.

Figure 2. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (A00248-1). CD147/Emmprin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD147/Emmprin Antibody (A00248-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (A00248-1). CD147/Emmprin was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD147/Emmprin Antibody (A00248-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Human CD147/Emmprin DyLight® 488 conjugated BSG Antibody
Boster
catalog: A00248-Dyl488
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Human CD147/Emmprin DyLight® 550 conjugated BSG Antibody
Boster
catalog: A00248-Dyl550
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-EMMPRIN (Q370) BSG Antibody
Boster
catalog: A00248-2
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Hu CD147 Purified BSG Monoclonal Antibody
Boster
catalog: M00248
mouse monoclonal (MEM-M6/1)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
quantity: 0.1 mg (C100)
price: 280 USD
to the supplier
Anti-CD147 BSG Rabbit Monoclonal Antibody
Boster
catalog: M00248-1
domestic rabbit monoclonal (AAOC-2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 µl
price: 315 USD
to the supplier
Anti-CD147 Rabbit Monoclonal Antibody
Boster
catalog: M00248-3
domestic rabbit monoclonal (26B41)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 µl/vial
price: 315 USD
to the supplier
Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 3B13G7)
Boster
catalog: M00248-4
mouse monoclonal (3B13G7)
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier
Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 7H5E7)
Boster
catalog: M00248-5
mouse monoclonal (7H5E7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier
Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 6H2B2)
Boster
catalog: M00248-6
mouse monoclonal (6H2B2)
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier