domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
reactivity: human, mouse, rat
application: western blot, flow cytometry
Anti-STAT5a antibody, PA1840, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: COLO320 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate.
Figure 2. Flow Cytometry analysis of U20S cells using anti-STAT5A antibody (PA1840). Overlay histogram showing U20S cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- STAT5A Antibody (PA1840,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 3. Flow Cytometry analysis of Jurkat cells using anti-STAT5A antibody (PA1840). Overlay histogram showing Jurkat cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- STAT5A Antibody (PA1840,1ug/1x10 6 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AOI-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of STAT5a in A431 cell lysate (M01087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody (Catalog # M01087-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (EDD-19)
reactivity: human, mouse
application: western blot, immunohistochemistry
reactivity: human, mouse
application: western blot, immunohistochemistry
Western blot analysis of Phospho-Stat5 (Y694) expression in A431 cell lysate treated with EGF (P01087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody (Catalog # P01087-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (HDO-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of STAT5A/B expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) PC12 cell lysate (M01087). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody (Catalog # M01087) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A
quantity: 100 µl
price: 315 USD
to the supplier