Anti-STAT3 Antibody Picoband™
Boster
catalog: PB9511
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Anti- STAT3 Picoband antibody, PB9511, Western blotting. All lanes: Anti STAT3 (PB9511) at 0.5ug/ml. Lane 1: Rat Lung Tissue Lysate at 50ug. Lane 2: Rat Brain Tissue Lysate at 50ug. Lane 3: Human Placenta Tissue Lysate at 50ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: PANC Whole Cell Lysate at 40ug. Lane 6: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 88KD. Observed bind size: 88KD
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Boster
catalog: P00007-2
domestic rabbit monoclonal (IFA-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody(P00007-2). STAT3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-STAT3 Antibody (P00007-2)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Phospho-STAT3 (Tyr705) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with IFN-a (P00007-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # P00007-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
quantity: 100 µl
price: 315 USD
to the supplier
Anti-STAT3 Rabbit Monoclonal Antibody
Boster
catalog: M00007-2
domestic rabbit monoclonal (GO-19)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of STAT3 expression in A431 cell lysate (M00007-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # M00007-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody
Boster
catalog: P00007-1
domestic rabbit monoclonal (CAA-19)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF (P00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # P00007-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
quantity: 100 µl
price: 315 USD
to the supplier
Anti-STAT3 Rabbit Monoclonal Antibody
Boster
catalog: M00007
domestic rabbit monoclonal (AIH-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Raji cell lysate (M00007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # M00007) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
quantity: 100 µl
price: 315 USD
to the supplier
Anti-STAT3 Rabbit Monoclonal Antibody
Boster
catalog: M00007-1
domestic rabbit monoclonal (CAB-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of STAT3 expression in Raji cell lysate (M00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # M00007-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
quantity: 100 µl
price: 315 USD
to the supplier
Anti-STAT3 Antibody
Boster
catalog: A00007-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 0.1 mg
price: 404 USD
to the supplier
Anti-STAT3 Antibody
Boster
catalog: A00007-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Stat3 (S691) Antibody
Boster
catalog: A00007S691
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Stat3 (phospho-S727) Antibody
Boster
catalog: A00007S727
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Stat3 (phospho-S727) Antibody
Boster
catalog: A00007S727-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 547 USD
to the supplier
Anti-STAT3 (Phospho-S727) Antibody
Boster
catalog: A00007S727-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Stat3 (T721) Antibody
Boster
catalog: A00007T721
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Stat3 (phospho-Y705) Antibody
Boster
catalog: A00007Y705
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Stat3 (phospho-Y705) Antibody
Boster
catalog: A00007Y705-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 547 USD
to the supplier
Anti-STAT3 (Phospho-Tyr705) Antibody
Boster
catalog: A00007Y705-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261
Boster
catalog: P00007-3
domestic rabbit monoclonal (RM261)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 uL
price: 399 USD
to the supplier