Anti-STAT1 Antibody
Boster
catalog: PA1075

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti-STAT1 antibody, PA1075, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate.

Anti-STAT1 antibody, PA1075, Western blotting. Lane 1: Mouse Heart Tissue Lysate. Lane 2: Mouse Liver Tissue Lysate. Lane 3: Mouse Brain Tissue Lysate. Lane 4: Mouse Kidney Tissue Lysate. Lane 5: Mouse Spleen Tissue Lysate. Lane 6: Mouse Thymus Tissue Lysate. Lane 7: Mouse Lung Tissue Lysate. Lane 8: Mouse Intestine Tissue Lysate. Lane 9: Mouse Ovary Tissue Lysate

Figure 3. Western blot analysis of STAT1 using anti- STAT1 antibody (PA1075). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, . Lane 2: rat thymus tissue lysates, . Lane 3: rat testis tissue lysates, . Lane 4: mouse testis tissue lysates, . Lane 5: mouse kidney tissue lysates, . Lane 6: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- STAT1 antigen affinity purified polyclonal antibody (Catalog # PA1075) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-STAT1 Antibody
Boster
catalog: PA1508

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-STAT1 antibody, PA1508, Western blotting. All lanes: Anti STAT1 (PA1508) at 0.5ug/ml. Lane 1: MM451 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: HT1080 Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 87KD. Observed bind size: 87KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-STAT1 Antibody Picoband™
Boster
catalog: PB9510

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti- STAT1 Picoband antibody, PB9510, Western blotting. All lanes: Anti STAT1 (PB9510) at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 50ug. Lane 2: Rat Brain Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: Human Placenta Tissue Lysate at 50ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 91KD(Alpha), 84KD(Beta ). Observed bind size: 91KD(Alpha), 84KD(Beta )

Anti- STAT1 Picoband antibody, PB9510, IHC(P). IHC(P): Mouse Intestine Tissue

Anti- STAT1 Picoband antibody, PB9510, IHC(P). IHC(P): Rat Intestine Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-STAT1 Antibody Picoband™
Boster
catalog: A00036-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human PC-3 whole cell lysates, . Lane 3: human Caco-2 whole cell lysates, . Lane 4: human A549 whole cell lysates, . Lane 5: human K562 whole cell lysates, . Lane 6: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # A00036-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.

Figure 2. IHC analysis of STAT1 using anti- STAT1 antibody (A00036-2). . STAT1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- STAT1 Antibody (A00036-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IF analysis of STAT1 using anti- STAT1 antibody (A00036-2) . STAT1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- STAT1 Antibody (A00036-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-STAT1 Antibody Picoband™ (monoclonal, 12C7)
Boster
catalog: M00036-2

mouse monoclonal (12C7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-STAT1 Rabbit Monoclonal Antibody
Boster
catalog: M00036-1

domestic rabbit monoclonal (BEE-19)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of STAT1 expression in HeLa cell lysate (M00036-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 monoclonal antibody (Catalog # M00036-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-STAT1 alpha Rabbit Monoclonal Antibody
Boster
catalog: M00036

domestic rabbit monoclonal (HBG-19)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of STAT1 alpha expression in Hela cell lysate (M00036). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 monoclonal antibody (Catalog # M00036) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal Antibody
Boster
catalog: P00036

domestic rabbit monoclonal (HHB-19)
reactivity: human
application: western blot

Western blot analysis of Phospho-STAT1 (Y701) expression in (1) A431 cell lysate; (2) A431 lysate cell treated with EGF (P00036). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 monoclonal antibody (Catalog # P00036) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1
quantity: 100 µl
price: 315 USD
to the supplier