domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
All lanes: Anti B Raf (PB9164) at 0.5ug/ml. WB: Recombinant Human B Raf Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD
All lanes: Anti B Raf (PB9164) at 0.5ug/ml. Lane 1: Rat Testis Tissue at 50ug. Lane 2: Rat Brain Tissue at 50ug. Lane 3: Mouse Testis Tissue at 50ug. Lane 4: Mouse Brain Tissue at 50ug. Lane 5: Hela Whole Cell Lysate at 40ug. Lane 6: Jurkat Whole Cell Lysate at 40ug. Lane 7: MCF-7 Whole Cell Lysate at 40ug. Lane 8: K562 Whole Cell Lysate at 40ug. Predicted bind size: 84KD . Observed bind size: 84KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (IBE-2)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation
Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA (P00075). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRAF monoclonal antibody (Catalog # P00075) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRAF
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (CCG-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody(M00075). BRAF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRAF Antibody (M00075)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of B Raf expression in (1)Human brain lysate (2)HepG2 cell lysate (M00075). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRAF monoclonal antibody (Catalog # M00075) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRAF
quantity: 100 µl
price: 315 USD
to the supplier