domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
Anti-SPARC antibody, PA1585, Western blotting. Lane 1: Recombinant Human SPARC Protein 10ng. Lane 2: Recombinant Human SPARC Protein 5ng. Lane 3: Recombinant Human SPARC Protein 2.5ng
Anti-SPARC antibody, PA1585, Western blotting. WB: HELA Cell Lysate
Anti-SPARC antibody, PA1585, IHC(P). IHC(P): Human Intestinal Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti- SPARC Picoband antibody, PB9559, Western blotting. All lanes: Anti SPARC (PB9559) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: 293T Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 35KD. Observed bind size: 35KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: rat testis tissue lysates, . Lane 3: mouse brain tissue lysates, . Lane 4: mouse testis tissue lysates, . Lane 5: U20s whole Cell lysates, . Lane 6: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 43KD. The expected band size for SPARC is at 35 KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates,. Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 37KD. The expected band size for SPARC is at 35KD.
quantity: 100µg/vial
price: 315 USD
to the supplier