rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, . Lane 2: mouse brain tissue lysate, . Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 antigen affinity purified polyclonal antibody (Catalog # PB9435) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.
Anti- SLC2A1 Picoband antibody, PB9435, IHC(P). IHC(P): Mouse Brain Tissue
Anti- SLC2A1 Picoband antibody, PB9435, IHC(P). IHC(P): Rat Brain Tissue
quantity: 100 ug/vial
price: 240 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Anti-SLC2A1 antibody, PA1120-1, Western blotting. All lanes: Anti SLC2A1(PA1120-1) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: SW620 Whole Cell Lysate at 40ug. Lane 3: 293T Whole Cell Lysate at 40ug. Predicted bind size: 57KD. Observed bind size: 57KD
quantity: 100 ug/vial
price: 240 USD
to the supplier
rabbit monoclonal (CGG-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
Immunofluorescent analysis of HepG2 cells, using GLUT1 Antibody .
Western blot analysis of GLUT1 expression in HepG2 lysate (M00163). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 monoclonal antibody (Catalog # M00163) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1
Immunohistochemical analysis of paraffin-embedded human cervix cancer, using GLUT1 Antibody(M00163). SLC2A1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC2A1 Antibody (M00163)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 299 USD
to the supplier
mouse monoclonal (10C10)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
