domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
Anti- CD62P antibody, PA1715, Western blotting. All lanes: Anti CD62P (PA1715) at 0.5ug/ml. WB: PANC Whole Cell Lysate at 40ug. Predicted bind size: 91KD. Observed bind size: 140KD
Anti- CD62P antibody, PA1715, IHC(P). IHC(P): Human Placenta Tissue
Anti- CD62P antibody, PA1715, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
Figure 1. IHC analysis of CD62P using anti-CD62P antibody (PB9363). CD62P was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD62P Antibody (PB9363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. Western blot analysis of CD62P using anti-CD62P antibody (PB9363). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate,. Lane 2: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (Catalog # PB9363) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62P at approximately 91KD. The expected band size for CD62P is at 140KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AAEC-19)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
mouse monoclonal (AK4)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry