domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
Anti- Syndecan-1 Picoband antibody, PB9743, Western blotting. All lanes: Anti Syndecan-1 (PB9743) at 0.5ug/ml. Lane 1: HT1080 Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: 293T Whole Cell Lysate at 40ug. Lane 4: HEPG2 Whole Cell Lysate at 40ug. Lane 5: HELA Whole Cell Lysate at 40ug. Predicted bind size: 32KD. Observed bind size: 50KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of Syndecan-1/SDC1 using anti-Syndecan-1/SDC1 antibody (A00991-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1/SDC1 antigen affinity purified polyclonal antibody (Catalog # A00991-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1/SDC1 at approximately 60KD. The expected band size for Syndecan-1/SDC1 is at 32KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
domestic rabbit monoclonal (AbS91)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry