Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)
Boster
catalog: MA1115
mouse monoclonal (AC-15)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 34

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: Rat Cardiac Muscle Tissue Lysate. Lane 4: Rat Brain Tissue Lysate. Lane 5: A453 Cell Lysate. Lane 6: HE

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Spleen Tissue Lysate. Lane 3: Rat Brain Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: HELA Cell Lysate. Lane 6: SMMC Cell

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P). IHC(P): Mouse Intestine Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-beta Actin ACTB Rabbit Monoclonal Antibody
Boster
catalog: M01263
domestic rabbit monoclonal (BF-1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1

Immunofluorescent analysis of Hela cells, using beta Actin Antibody

Immunohistochemical analysis of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart, using beta Actin Antibody(M01263). ACTB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTB Antibody (M01263)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate (M01263). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTB monoclonal antibody (Catalog # M01263) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTB
quantity: 100 µl
price: 315 USD
to the supplier
Anti-beta Actin ACTB Antibody
Boster
catalog: A01263
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of beta Actin expression in MW marker (lane 1), human embryonic kidney 293 cell lysates (lane 2), human lung carcinoma A549 cell lysates (lane 3) and mouse brain extract (lane 4). Load: 35 µg per lane. Beta Actin at 42 kDa (arrowhead) was detected using rabbit anti- beta Actin affinity purified polyclonal antibody (Catalog # A01263) at 1:1,500.

Beta Actin was detected in paraffin-embedded sections of rana pipiens tissues using rabbit anti- beta Actin affinity purified polyclonal antibody (Catalog # A01263) at 1:200. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
quantity: 200 ug
price: 404 USD
to the supplier
Anti-Beta-actin ACTB Antibody
Boster
catalog: A01263-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry
quantity: 0.1 mg
price: 404 USD
to the supplier
Anti-beta-Actin ACTB Antibody
Boster
catalog: A01263-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 0.1 mg
price: 404 USD
to the supplier
Anti-beta-Actin ACTB Antibody (biotin)
Boster
catalog: A01263-Biotin
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA
quantity: 0.1 mg
price: 404 USD
to the supplier
Anti-beta-Actin ACTB Antibody (HRP)
Boster
catalog: A01263-HRP
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA
quantity: 0.1 mg
price: 404 USD
to the supplier
Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated)
Boster
catalog: H01263
mouse monoclonal (17A12)
reactivity: human
application: western blot
quantity: 100 µl/vial
price: 315 USD
to the supplier
Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112
Boster
catalog: M01263-2
domestic rabbit monoclonal (RM112)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
quantity: 100 uL
price: 399 USD
to the supplier
Anti-Beta-Actin Antibody
Boster
catalog: M01263-3
mouse monoclonal (monoclonal)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 80 ul
price: 173 USD
to the supplier
Anti-beta Actin Mouse Monoclonal Antibody
Boster
catalog: M01263-4
mouse monoclonal (17A10)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 µl/vial
price: 315 USD
to the supplier
Anti-beta Actin Mouse Monoclonal Antibody
Boster
catalog: M01263-5
mouse monoclonal (17A11)
reactivity: human
application: western blot
quantity: 100 µl/vial
price: 315 USD
to the supplier
Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin)
Boster
catalog: M01263-Biotin
mouse monoclonal (10B7)
reactivity: human
application: western blot, ELISA
quantity: 0.1 mg
price: 404 USD
to the supplier