Anti-Bcl-XL/BCL2L1 Antibody Picoband™
Boster
catalog: A00181

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,. Lane 2: NIH3T3 whole cell lysates,. Lane 3: HEPA1-6 whole cell lysates,. Lane 4: SGC7901 whole cell lysates,. Lane 5: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XL antigen affinity purified polyclonal antibody (Catalog # A00181) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-XL at approximately 30KD. The expected band size for Bcl-XL is at 26KD.

Figure 2. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Bcl-XL was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bcl-XL Antibody (A00181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Bcl-XL was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bcl-XL Antibody (A00181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bcl-XS/BCL2L1 Antibody
Boster
catalog: PA1232

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-Bcl-XS antibody, PA1232, Western blotting. All lanes: Anti Bcl-XS (PA1232) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Predicted bind size: 19KD. Observed bind size: 19KD

Anti-Bcl-XS antibody, PA1232, IHC(P). IHC(P): Human Colon Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bcl-X/BCL2L1 Antibody Picoband™
Boster
catalog: PB9917

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of Bcl-X using anti- Bcl-X antibody (PB9917). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Bcl-X antigen affinity purified polyclonal antibody (Catalog # PB9917) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-X at approximately 29 KD, 60KD. The expected band size for Bcl-X is at 26KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
Boster
catalog: M00181-1

domestic rabbit monoclonal (AOD-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human liver cancer, using Bcl-XL Antibody(M00181-1). BCL2L1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2L1 Antibody (M00181-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Bcl-XL expression in (1) K562 cell lysate; (2) C6 cell lysate (M00181-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2L1 monoclonal antibody (Catalog # M00181-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2L1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody
Boster
catalog: A00181-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human Bcl-XL DyLight® 550 conjugated BCL2L1 Antibody
Boster
catalog: A00181-Dyl550

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier