Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)
Boster
catalog: MA1004

mouse monoclonal (Bcl-2-100)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1

Anti-Bcl-2 antibody (monoclonal), MA1004, Western blotting. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Spleen Tissue Lysate. Lane 3: Rat Small Intestine Tissue Lysate. Lane 4: Rat Liver Tissue Lysate.

Anti-Bcl-2 antibody (monoclonal), MA1004, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-Bcl-2 antibody (monoclonal), MA1004, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bcl2 alpha Antibody
Boster
catalog: RP1003

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Figure. Western blot analysis of BCL-2 using anti- BCL-2 antibody (RP1003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human Bcl-2 Protein 0.5ng,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- BCL-2 antigen affinity purified polyclonal antibody (Catalog # RP1003) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL-2 at approximately 26KD. The expected band size for BCL-2 is at 26KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Bcl-2/BCL2 Antibody Picoband™
Boster
catalog: A00040-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (A00040-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-2 antigen affinity purified polyclonal antibody (Catalog # A00040-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-2 at approximately 26KD. The expected band size for Bcl-2 is at 26KD.

Figure 2. IHC analysis of Bcl-2 using anti-Bcl-2 antibody (A00040-2). Bcl-2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bcl-2 Antibody (A00040-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Bcl-2 Rabbit Monoclonal Antibody
Boster
catalog: M00040-1

domestic rabbit monoclonal (HF-2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody(M00040-1). BCL2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2 Antibody (M00040-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Bcl-2 expression in (1) Jurkat cell lysate;(2) MCF-7 cell lysate (M00040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 monoclonal antibody (Catalog # M00040-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Bcl-2 Rabbit Monoclonal Antibody
Boster
catalog: M00040-2

domestic rabbit monoclonal (AAOH-2)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of Bcl-2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M00040-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 monoclonal antibody (Catalog # M00040-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Human Bcl-2 DyLight® 488 conjugated Antibody
Boster
catalog: A00040-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-Human Bcl-2 DyLight® 550 conjugated Antibody
Boster
catalog: A00040-Dyl550

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier