mouse monoclonal (DCS-6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti- Cyclin D1 Picoband antibody, PB9370, Western blotting. All lanes: Anti Cyclin D1 (PB9370) at 0.5ug/ml. Lane 1: Human Placenta Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: 293T Whole Cell Lysate at 40ug. Predicted bind size: 33KD. Observed bind size: 33KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
domestic rabbit monoclonal (DAD-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of Cyclin D1 expression in (1)MCF-7 cell lysates;(2) LnCaP cell lysates (M00149-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND1 monoclonal antibody (Catalog # M00149-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCND1
Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody(M00149-1). CCND1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCND1 Antibody (M00149-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IF analysis of immunocytochemical section of MCF-7 cells using anti- Cyclin D1 antibody (M00149-1) . Cyclin D1 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Cyclin D1 Antibody (M00149-1) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry
reactivity: human, rat
application: western blot, flow cytometry
Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (PA1245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate,. Lane 2: Human Placenta Tissue Lysate,. Lane 3: Rat Brain Tissue Lysate,. Lane 4: MCF-7 Whole Cell Lysate,. Lane 5: COLO320 Whole Cell Lysate,. Lane 6: SW620 Whole Cell Lysate,. Lane 7: MM231 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # PA1245-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 33KD. The expected band size for Cyclin D1 is at 33KD.
Figure 2. Flow Cytometry analysis of U-87MG cells using anti- Cyclin D1 antibody (PA1245-2). Overlay histogram showing U-87MG cells stained with PA1245-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (PA1245-2,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100μg/vial
price: 315 USD
to the supplier