domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti-Rab5 antibody, PA1773, Western blotting. Lane 1: Rat Brain Tissue Lysate . Lane 2: Rat Ovary Tissue Lysate . Lane 3: Human Placenta Tissue Lysate . Lane 4: HELA Cell Lysate . Lane 5: 293T Cell Lysate . Lane 6: A375 Cell Lysate . Lane 7: COLO320 Cell Lysate. Lane 8: MM453 Cell Lysate
Anti-Rab5 antibody, PA1773, IHC(P). IHC(P):Rat Brain Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti-Rab5 Picoband antibody, PB9787, Western blotting. All lanes: Anti Rab5 (PB9787) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: 293T Whole Cell Lysate at 40ug. Lane 6: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 24KD. Observed bind size: 24KD
Anti-Rab5 Picoband antibody, PB9787,IHC(P). IHC(P): Human Intestinal Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (EOE-18)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Immunohistochemical analysis of paraffin-embedded human colon, using Rab5 Antibody(M01891). RAB5A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RAB5A Antibody (M01891)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Rab5 expression in MCF-7 cell lysate (M01891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A monoclonal antibody (Catalog # M01891) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5A
quantity: 100 µl
price: 315 USD
to the supplier
mouse monoclonal (3000000000)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section