domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 1
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 1
Figure 1. Western blot analysis of Bax using anti-Bax antibody (A00183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates,. Lane 2: mouse thymus tissue lysates,. Lane 3: HEPA1-6 whole cell lysates,. Lane 4: HELA whole cell lysates,. Lane 5: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # A00183) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21KD. The expected band size for Bax is at 21KD.
Figure 2. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AAB-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
Western blot analysis of Bax in (1) HeLa cell lysate;(2) RAW2647 Whole Cell Lysate (M00183-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAX monoclonal antibody (Catalog # M00183-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAX
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody(M00183-1). BAX was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BAX Antibody (M00183-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry
reactivity: human
conjugate: DyLight488
application: flow cytometry
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry