Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)
Boster
catalog: MA1000

mouse monoclonal (AC-40)
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry

Anti- Actin antibody, MA1000, IHC(F). IHC(F): Mouse Intestine Tissue

Anti- Actin antibody, MA1000, IHC(F). IHC(F): Mouse Spleen Tissue

Anti- Actin antibody, MA1000, IHC(F). IHC(F): Rat Intestine Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Actin/ACTA1 Antibody Picoband™
Boster
catalog: PB9751

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti- Actin Picoband antibody, PB9751, Western blotting. All lanes: Anti Actin (PB9751) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: Mouse Spleen Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 42KD. Observed bind size: 42KD

Anti- Actin Picoband antibody, PB9751,IHC(P). IHC(P): Mouse Spleen Tissue

Anti- Actin Picoband antibody, PB9751,IHC(P). IHC(P): Rat Spleen Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Actin/ACTA1 Antibody
Boster
catalog: RP1070

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti- ACTIN antibody, RP1070, Western blotting. All lanes: Anti ACTIN (RP1070) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Rat Kidney Tissue Lysate at 50ug. Lane 3: Mouse Spleen Tissue Lysate at 50ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Lane 7: A549 Whole Cell Lysate at 40ug. Predicted bind size: 42KD. Observed bind size: 42KD

Anti- ACTIN antibody, RP1070, IHC(P). IHC(P): Mouse Intestine Tissue

Anti- ACTIN antibody, RP1070, IHC(P). IHC(P): Rat Spleen Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Boster
catalog: M02014-1

domestic rabbit monoclonal (BE-1)
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Actin expression in (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate (M02014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1

Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody(M02014-1). ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of Hela cells using anti- Actin antibody (M02014-1) . Actin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Actin Antibody (M02014-1) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody
Boster
catalog: M02014-4

domestic rabbit monoclonal (C-1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate (M02014-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody(M02014-4). ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-4)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of Hela cells using anti- Actin (Alpha-Actin) antibody (M02014-4) . Actin (Alpha-Actin) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Actin (Alpha-Actin) Antibody (M02014-4) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Boster
catalog: M02014-2

domestic rabbit monoclonal (AAOF-1)
reactivity: human
application: western blot, immunoprecipitation

quantity: 100 µl
price: 315 USD
to the supplier

Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Boster
catalog: M02014-3

domestic rabbit monoclonal (AAOG-1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 µl
price: 315 USD
to the supplier

Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5)
Boster
catalog: M02014-5

mouse monoclonal (3H5)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier